
BEGINNERS LEVEL
THE BASICS:
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What are nucleic acids?
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DNA structure and functions
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Genomic DNA and cDNA
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Why do we need to do PCR?
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Components of PCR and their roles
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Different temperatures for PCR
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Calculating the melting temperature
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Methodology
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Detection of PCR products or amplicons
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Quantification of extracted RNA or DNA
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Reasons for PCR failures
REAL-TIME PCR:
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Introduction
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Principles and chemistries
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Advantages vis-a-vis conventional traditional PCR
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Quality of RNA and DNA
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Amplification of products
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High resolution melt curves and their uses