BEGINNERS LEVEL

THE BASICS:

• What are nucleic acids?

• DNA structure and functions

• Genomic DNA and cDNA

• Why do we need to do PCR?

• Components of PCR and their roles

• Different temperatures for PCR

• Calculating the melting temperature

• Methodology

• Detection of PCR products or amplicons

• Quantification of extracted RNA or DNA

• Reasons for PCR failures

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REAL-TIME PCR:

• Introduction

• Principles and chemistries

• Advantages vis-a-vis conventional traditional PCR

• Quality of RNA and DNA

• Amplification of products

• High resolution melt curves and their uses