BEGINNERS LEVEL

THE BASICS:

  • What are nucleic acids?

  • DNA structure and functions

  • Genomic DNA and cDNA

  • Why do we need to do PCR?

  • Components of PCR and their roles

  • Different temperatures for PCR

  • Calculating the melting temperature

  • Methodology

  • Detection of PCR products or amplicons

  • Quantification of extracted RNA or DNA

  • Reasons for PCR failures

REAL-TIME PCR:

  • Introduction

  • Principles and chemistries

  • Advantages vis-a-vis conventional traditional PCR

  • Quality of RNA and DNA

  • Amplification of products

  • High resolution melt curves and their uses